Patanjali’s Renogrit ranked among top 100 research papers of 2024 in Nature Portfolio journal | File Photo
National/ Haridwar, March 18: Inspired by Yog Rishi Swami Ramdev Ji Maharaj and evidence-based Ayurvedic medicine research conducted by scientists of Patanjali, the research paper on herbal formulation Renogrit has been featured among the top 100 research papers of 2024 in the “Scientific Reports” journal of prestigious “Nature Portfolio” publication.
With an Impact Factor of 3.8, Scientific Reports is the 5th most-cited journal globally. The research paper on Renogrit has been downloaded 2,568 times, reaffirming the growing global interest in Ayurvedic medicines, not only as effective remedies for various ailments but also as a subject of scientific curiosity because it is fascinating how a simple herbal formulation can combat severe diseases without any adverse effects.
Patanjali’s Ayurvedic formulation, Renogrit, has demonstrated its potential not only in repairing kidney damage caused by the allopathic anti-cancer drug, cisplatin but also in alleviating oxidative stress on kidney cells.
On this occasion, Acharya Balkrishna stated that the success of Renogrit is a significant step in establishing the scientific credibility of Ayurveda on a global scale. It demonstrates that when ancient wisdom is tested on modern scientific parameters, it yields groundbreaking results.
To read this research paper in detail, click on the link: https://www.nature.com/articles/s41598-024-69797-3
Figure 13. Representation of the molecular changes induced by cisplatin in HK-2 cells and in C. elegans along with the subsequent therapeutic modulation by Renogrit.
As 8 mm band using spray-on technique on a TLC plate. The plate was developed using CAMAG twin trough chamber pre-saturated (10 min) with mobile phase (Toluene: ethyl acetate: methanol: formic acid (5:4:1:0.2 v/v/v/v). TLC plate was developed up to 70 mm, dried under warm air, and visualized under UV 254 nm. The image was documented and the same TLC was scanned at 280 nm, slit dimension was 6 x 0.45 mm, scanning speed was 20 mm/s, with data resolution of 100 um/step. A deuterium lamp was used under absorption mode.
For quantification, several concentrations of each standard solution were applied on the plate and on the basis of linearity plot concentration of specific phytochemical, concentration of the same phytochemical in Renogrit was evaluated under same chromatographic conditions.
In order to rule out any deviations in the quantity of phytometabolites characterized by HPTLC, further analysis of phytometabolites was performed by UHPLC. Three different batches of Renogrit were evaluated to generate a thorough quantitative phytochemical profile. UHPLC-based analysis of phytocompounds was evaluated on Prominence-XR UHPLC system (Shimadzu, Japan fitted with Quaternary pump (Nexera XR LC-20AD XR), diode array detector (DAD SPD-M20 A), auto-sampler (Nexera XR SIL-20 AC XR), degassing unit (DGU-20A 5R) and column oven (CTO-10 AS VP). Separation was achieved using a Shodex C18-4E (5 um, 4.6 × 250 mm) column subjected to binary gradient elution. The two solvents used for the analysis consisted of water comprising 0.1% acetic acid (solvent A) and acetonitrile (solvent B).
Gradient programming of the solvent system was primarily at 0-5% B for 0-10 min, 5-10% B from 10 to 20 min, 10-20% B from 20 to 30 min, 20-30% B from 30 to 40 min, 30-50% B from 40 to 50 min, 50-70% B from 50 to 60 min, 70-90% B from 60 to 70 min, 90-0% B from 70 to 72 min, 0% B from 72 to 75 min with a flow rate of 1 ml/min. 10 ul of standard and test solution were injected, the column oven temperature was maintained at 35 °C, and the detector wavelength was set at 270 nm throughout the analysis. Data were presented as mean + S.E.M (n = 3).
